The human intrinsic factor-vitamin B12 receptor, cubilin: molecular characterization and chromosomal mapping of the gene to 10p within the autosomal recessive megaloblastic anemia (MGA1) region
| Autor: | R, Kozyraki, M, Kristiansen, A, Silahtaroglu, C, Hansen, C, Jacobsen, N, Tommerup, P J, Verroust, S K, Moestrup |
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| Rok vydání: | 1998 |
| Předmět: |
Furin
Intrinsic Factor DNA Complementary Kidney Cortex Anemia Megaloblastic Sequence Homology Amino Acid Chromosomes Human Pair 10 Swine Molecular Sequence Data Chromosome Mapping Genes Recessive Receptors Cell Surface Hybrid Cells Polymerase Chain Reaction Rats Vitamin B 12 Genes Species Specificity Animals Humans Amino Acid Sequence Subtilisins Chromosomes Artificial Yeast In Situ Hybridization Fluorescence |
| Zdroj: | Blood. 91(10) |
| ISSN: | 0006-4971 |
| Popis: | Uptake of vitamin B12 (cyanocobalamin) is facilitated by the cobalamin-binder gastric intrinsic factor (IF), which recognizes a 460-kD receptor, cubilin, present in the epithelium of intestine and kidney. Surface plasmon resonance analysis of ligand-affinity-purified human cubilin demonstrated a high-affinity calcium- and cobalamin-dependent binding of IF-cobalamin. Complete cDNA cloning of the human receptor showed a 3597 amino acid peripheral membrane protein with 69% identity to rat cubilin. Amino-terminal sequencing of the receptor indicates that the cDNA sequence encodes a precursor protein undergoing proteolytic processing due to cleavage at a recognition site (Arg7-Glu8-Lys9-Arg) for the trans-Golgi proteinase furin. Using fluorescence in situ hybridization, radiation hybrid mapping, and screening of YAC clones, the human cubilin gene was mapped between the markers D10S1661 and WI-5445 on the short arm of chromosome 10. This is within the autosomal recessive megaloblastic anemia (MGA1) 6-cM region harboring the unknown recessive-gene locus of juvenile megaloblastic anemia caused by intestinal malabsorption of cobalamin (Imerslund-Gräsbeck's disease). In conclusion, the present molecular and genetic information on human cubilin now provides circumstantial evidence that an impaired synthesis, processing, or ligand binding of cubilin is the molecular background of this hereditary form of megaloblastic anemia. |
| Databáze: | OpenAIRE |
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