| Autor: |
Aishwarya, Sridharan, S Y, John Lim, Graham D, Wright, Leah A, Vardy |
| Rok vydání: |
2019 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2109 |
| ISSN: |
1940-6029 |
| Popis: |
The study of skin pigmentation requires determining the rate of melanin production in melanocytes and quantifying the rate of melanosome transfer to keratinocytes. Here, we describe a method to quantify melanosome transfer using immunofluorescence microscopy coupled with automated image analysis of in vitro human melanocytes and keratinocytes in co-culture. In this method, the number of melanin capped keratinocyte nuclei is quantified. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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