| Autor: |
Jalil, Normala, Victor Tosin, Okomoda, Azizul Alim, Mohd, Asma Ariffin, Nur, Ambok Bolong, Abol-Munafi, Shahreza, Md Sheriff |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
Veterinary Sciences |
| ISSN: |
2306-7381 |
| Popis: |
This study was designed to examine the use of RAPD markers in discriminating triploid and diploid African catfish Clarias gariepinus (Burchell, 1822). Following a routine technique, triploidy was induced by cold shock and confirm by erythrocyte measurement in C. gariepinus. Thereafter, 80 RAPD markers were screened; out of which, three showed the highest percentage of polymorphism (i.e., OPB 16 = 71.43%; OPC 14 = 61.9%; OPD 12 = 75%). The results obtained showed genotype differences between triploid and diploid without overlapping. However, the development of a Sequence Characterized Amplified Region (SCAR) marker was not achievable because progenies of triploid and diploid C. gariepinus could not be differentiated based on a specific fragment. Consequently, the genetic distance showed high similarities for both treatments and the UPGMA-generated dendrogram could not separate the treatments into two distinct clusters. It was concluded that RAPD makers cannot be used to separate the ploidy status of fishes. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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