| Autor: |
L V, Koval'chuk, L V, Gankovskaia, M V, Mezentseva, I M, Shapoval, Iu V, Aitova, A M, Magomedova, V V, Romanovskaia |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
Zhurnal mikrobiologii, epidemiologii i immunobiologii. (2) |
| ISSN: |
0372-9311 |
| Popis: |
Study of expression of cytokine genes in the process of cultivation of healthy donor leukocytes.RNA isolated before, after 3 and 24 hours of cultivation of leukocytes of 15 healthy donors aged 19 - 32 years was the object of the study. Study of the expression of genes of 8 cytokines by the level of their mRNA was performed by using polymerase chain reactionwith reverse transcription.In healthy donors among with individual differences, general regularities were traced: higher levels of IFNalpha and anti-inflammatory cytokine gene expression, as well as predominantly low expression of pro-inflammatory cytokines.In most of the cases in the process of cultivation of leukocytes isolated from blood donors the highest level of gene expression of pro-inflammatory cytokines is noted after 3 hours of cultivation, and expression of genes of anti-inflammatory cytokines remains constant for 24 hours of cultivation. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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