| Autor: |
V Y, Sekova, N N, Gessler, E P, Isakova, A N, Antipov, D I, Dergacheva, Y I, Deryabina, E V, Trubnikova |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
Prikladnaia biokhimiia i mikrobiologiia. 51(6) |
| ISSN: |
0555-1099 |
| Popis: |
In this study we investigated the activities of antioxidant enzymes (superoxide dismutases (SODs) and catalases (CATs)) and the ROS level in cells of Yarrowia lipolytica yeasts grown in a medium with different pH values (4.5, 5.5 and 9.0). It was shown that an increase in the cellular ROS level took place under both acid and alkaline conditions. The growth under extreme conditions was accompanied by a significant increase of SOD activity (by 2.5 times in the acid medium and by 4 times in the alkaline medium), but catalase activity did not change. A study of the electrophoretic profile of catalases showed the presence of three isoforms differing in inhibitor resistance. The electrophoretic profiles of SODs and their inhibitory analysis revealed there are two other isoforms, probably of mitochondrial origin, in addition to Cu and Zn SOD. The role of SOD in pH-adaptation of extremophilic Y. lipolytica yeasts is discussed. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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