| Autor: |
B, Goins, R, Klipper, A S, Rudolph, R O, Cliff, R, Blumhardt, W T, Phillips |
| Rok vydání: |
1993 |
| Předmět: |
|
| Zdroj: |
Journal of nuclear medicine : official publication, Society of Nuclear Medicine. 34(12) |
| ISSN: |
0161-5505 |
| Popis: |
We have recently developed a procedure to label liposomes containing reduced glutathione (GSH) with 99mTc using the lipophilic chelator, hexamethylpropyleneamine oxime (HMPAO). In the present study, we evaluated the use of 99mTc-liposomes to detect focal infection sites in rats. Rats were infected in the thigh by intramuscular injection with Staphylococcus aureus followed 24 hr later by an intravenous injection of 99mTc-liposomes, 67Ga-citrate, or 99mTc-human serum albumin (HSA). The animals were imaged under a gamma camera and subsequently killed at 4, 24 or 48 hr for tissue biodistribution studies. In contrast to infected rats receiving 67Ga-citrate or 99mTc-HSA, abscesses were prominently localized within 2 hr in rats after 99mTc-liposome injection, and continued to increase in activity up to 24 hr. Abscess-to-muscle ratios calculated from 24-hr biodistribution data obtained from tissue sampling were 35.3 +/- 7.6 for 99mTc-liposomes, 4.1 +/- 0.7 for 67Ga-citrate and 8.0 +/- 1.0 for 99mTc-HSA. These studies show the potential of using 99mTc-liposomes to localize infection. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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