Electrochemical Detection for Isothermal Loop-Mediated Amplification of Pneumolysin Gene of

Autor: Andrea, González-López, María Dolores, Cima-Cabal, Pablo, Rioboó-Legaspi, Estefanía, Costa-Rama, María Del Mar, García-Suárez, M Teresa, Fernández-Abedul
Rok vydání: 2022
Předmět:
Zdroj: Analytical chemistry. 94(38)
ISSN: 1520-6882
Popis: A highly sensitive electrochemical methodology for end-point detection of loop-mediated isothermal nucleic acid amplification reactions was developed. It is based on the oxidation process of phenol red (PR), commonly used as a visual indicator. The dependence of its redox process on pH, which changes during amplification, allows performing quantitative measurements. Thus, the change in the oxidation potential of PR during the amplification is used, for the first time, as the analytical signal that correlates with the number of initial DNA copies. As a proof-of-concept, the amplification of the pneumolysin gene from
Databáze: OpenAIRE
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