Intracellular recordings with patch-clamp pipettes from neurons; technical problems in analysis of conductance changes during brief oxygen deprivation of hippocampal slices

Autor:	G, Czéh, J, Czopf
Rok vydání:	1993
Předmět:	Electrophysiology Neurons Oxygen Pyramidal Cells Neural Conduction Animals In Vitro Techniques Hippocampus Sodium Channels Membrane Potentials Rats
Zdroj:	Neurobiology (Budapest, Hungary). 1(4)
ISSN:	1216-8068
Popis:	Brief oxygen deprivation causes reversible, rapid and large scale redistribution of ions in hippocampal slice preparations. Intracellular recordings revealed remarkable increases of membrane conductance in neurons restricted for the period of hypoxic spreading depression (SD). Voltage-clamp technique was applied to explore further details of the SD-associated conductance changes. Patch-clamp recording in whole-cell configuration allowed better control of membrane potentials. High resolution current-voltage (I-V) plots were obtained by ramp command technique. Limitations of the techniques and various test protocols are evaluated.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::3e6d8f2f0750f8c371c0531341293390 https://pubmed.ncbi.nlm.nih.gov/8069292 Zobrazit plný text záznamu