[Haplotype and heteroplasmy in the HV II region of mtDNA in human blood detected by PCR-DGGE]

Autor:	Xian-dun, Zhai, Dai-xin, Huang, Hui, Yin, Qin-en, Yang
Rok vydání:	2007
Předmět:	China Genetic Heterogeneity Genetics Population Asian People Haplotypes Mutation Humans Electrophoresis Polyacrylamide Gel Sequence Analysis DNA DNA Mitochondrial Polymerase Chain Reaction
Zdroj:	Fa yi xue za zhi. 23(1)
ISSN:	1004-5619
Popis:	To establish a simple and effective technique for detecting haplotype and heteroplasmy of mtDNA, and investigate their frequencies in Chinese Han population.The fragments from 29-290 nt of mtDNA HV II from peripheral leukocytes of 200 unrelated Wuhan Han individuals were analyzed by using PCR-DGGE technique.Seventeen haplotypes were found in the range of 29-290 nt, and the haplotype diversity (HD) was 0.8844. The heterogeneity was observed from 4 individuals, and its frequency was 2%.PCR-DGGE is a simple, sensitive and effective technique in analyzing polymorphism and heteroplasmy of mtDNA, and can be used in forensic practice.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::3e43d9ba0649b914a92aa69104ef6272 https://pubmed.ncbi.nlm.nih.gov/17330756 Zobrazit plný text záznamu