| Autor: |
Ding-Ding, Zhang, Dan-Dan, Wang, Zhang, Wang, Yang-Bin, Wang, Guo-Xi, Li, Gui-Rong, Sun, Ya-Dong, Tian, Rui-Li, Han, Zhuan-Jian, Li, Rui-Rui, Jiang, Xiao-Jun, Liu, Xiang-Tao, Kang, Hong, Li |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
International Journal of Molecular Sciences |
| ISSN: |
1422-0067 |
| Popis: |
Few studies have been conducted regarding the biological function and regulation role of gga-miR-221-5p in the liver. We compared the conservation of miR-221-5p among species and investigated the expression pattern of gga-miR-221-5p, validating the direct target genes of gga-miR-221-5p by dual luciferase reporter assay, the biological function of gga-miR-221-5p in the liver was studied by gga-miR-221-5p overexpression and inhibition. Furthermore, we explored the regulation of gga-miR-221-5p and its target genes by treatment with estrogen and estrogen antagonists in vivo and in vitro. The results showed that miR-221-5p was highly conserved among species, expressed in all tested tissues and significantly downregulated in peak-laying hen liver compared to pre-laying hen liver. Gga-miR-221-5p could directly target the expression of elongase of very long chain fatty acids 6 (ELOVL6) and squalene epoxidase (SQLE) genes to affect triglyceride and total cholesterol content in the liver. 17β-estradiol could significantly inhibit the expression of gga-miR-221-5p but promote the expression of ELOVL6 and SQLE genes. In conclusion, the highly conservative gga-miR-221-5p could directly target ELOVL6 and SQLE mRNAs to affect the level of intracellular triglyceride and total cholesterol. Meanwhile, 17β-estradiol could repress the expression of gga-miR-221-5p but increase the expression of ELOVL6 and SQLE, therefore promoting the synthesis of intracellular triglyceride and cholesterol levels in the liver of egg-laying chicken. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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