RecG controls DNA amplification at double-strand breaks and arrested replication forks
| Autor: | Benura, Azeroglu, David R F, Leach |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
DNA Replication
Recombination Genetic Endodeoxyribonucleases DNA Repair Escherichia coli Proteins DNA Helicases Gene Amplification Recombinational DNA Repair Review Article Models Biological DNA amplification Bacterial Proteins double‐strand break repair Escherichia coli Animals Humans RecG DNA Breaks Double-Stranded Protein Multimerization Focus on… DNA Recombination | Genome organization and stability Review Articles |
| Zdroj: | Febs Letters |
| ISSN: | 1873-3468 |
| Popis: | DNA amplification is a powerful mutational mechanism that is a hallmark of cancer and drug resistance. It is therefore important to understand the fundamental pathways that cells employ to avoid over‐replicating sections of their genomes. Recent studies demonstrate that, in the absence of RecG, DNA amplification is observed at sites of DNA double‐strand break repair (DSBR) and of DNA replication arrest that are processed to generate double‐strand ends. RecG also plays a role in stabilising joint molecules formed during DSBR. We propose that RecG prevents a previously unrecognised mechanism of DNA amplification that we call reverse‐restart, which generates DNA double‐strand ends from incorrect loading of the replicative helicase at D‐loops formed by recombination, and at arrested replication forks. |
| Databáze: | OpenAIRE |
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