Rapid diagnosis of Venezuelan equine encephalomyelitis by fluorescence microscopy

Autor:	G A, Erickson, C J, Maré
Rok vydání:	1975
Předmět:	Encephalomyelitis Equine Goats Brain Fluorescent Antibody Technique Haplorhini Fibroblasts Kidney Encephalitis Virus Venezuelan Equine Mice Ducks Cricetinae Animals Horse Diseases Horses Cells Cultured
Zdroj:	American journal of veterinary research. 36(2)
ISSN:	0002-9645
Popis:	Goat Venezuelan equine encephalomyelitis (VEE) antiserum and normal serum were conjugated and evaluated for staining sensitivity and specificity. Cross-staining with either eastern or western equine encephalomyelitis virus-infected cells did not occur. The baby hamster kidney (BHK-21) cell line when combined with highly specific VEE conjugate detected 100 medium suckling mouse intracerebral lethal doses (suckling mouse LD-50/IC) of the 1B subtype of VEE virus per milliliter of equine tissue suspension. Conjugated goat antiserum was assayed for sensitivity for detection of VEE virus-infected equine serums and tissue suspensions. The BHK-21 cell line was superior to either primary duck embryo fibroblast (DEF) cells or African green monkey kidney (Vero) cells for propagating the GJ9-1BJ subtype of VEE virus.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::3de2cb6911fbd7a3db1fcfcbe9bb6f97 https://pubmed.ncbi.nlm.nih.gov/803352 Zobrazit plný text záznamu