| Autor: |
Esben G, Poulsen, Sofie V, Nielsen, Elin J, Pietras, Jens V, Johansen, Cornelia, Steinhauer, Rasmus, Hartmann-Petersen |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 1449 |
| ISSN: |
1940-6029 |
| Popis: |
The ubiquitin-proteasome system is the major pathway for intracellular protein degradation in eukaryotic cells. Due to the large number of genes dedicated to the ubiquitin-proteasome system, mapping degradation pathways for short lived proteins is a daunting task, in particular in mammalian cells that are not genetically tractable as, for instance, a yeast model system. Here, we describe a method relying on high-throughput cellular imaging of cells transfected with a targeted siRNA library to screen for components involved in degradation of a protein of interest. This method is a rapid and cost-effective tool which is also highly applicable for other studies on gene function. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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