| Autor: |
Shaimaa, Abulmagd, Abd El-Nasser A, Khattab, Hamdallah, Zedan |
| Rok vydání: |
2021 |
| Zdroj: |
Clinical and experimental vaccine research. 11(1) |
| ISSN: |
2287-3651 |
| Popis: |
In the present study, whole diphtheria toxin (dt) and fragment B (dtb) genes fromThe dt and dtb genes were isolated from bacterial strain ATCC (American Type Culture Collection) no. 13812. Plasmid pET29a+ was extracted by DNA-spin TM plasmid purification kit where genes were inserted using BamHI and HindIII-HF. Cloned pET29a+dt and pET29a+dtb plasmids were transformed intoThe genetic transformation ofThis study concluded with success in preparing genetically modified two strains for the production of a diphtheria vaccine, and to reach ideal production conditions to achieve the highest productivity. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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