Delivery of multiple CD8 cytotoxic T cell epitopes by DNA vaccination
| Autor: | S A, Thomson, M A, Sherritt, J, Medveczky, S L, Elliott, D J, Moss, G J, Fernando, L E, Brown, A, Suhrbier |
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| Rok vydání: | 1998 |
| Předmět: |
Cytotoxicity
Immunologic Thymoma Molecular Sequence Data Epitopes T-Lymphocyte Hemagglutinin Glycoproteins Influenza Virus Vaccinia virus Lymphocyte Activation Injections Intramuscular Lymphocyte Depletion Mice Vaccines DNA Animals Humans Amino Acid Sequence Mice Inbred BALB C Vaccines Synthetic Vaccination Antibodies Monoclonal Granulocyte-Macrophage Colony-Stimulating Factor T-Lymphocytes Helper-Inducer Mice Inbred C57BL Influenza A virus Antibody Formation Epitopes B-Lymphocyte Female Immunologic Memory Neoplasm Transplantation Plasmids T-Lymphocytes Cytotoxic |
| Zdroj: | Journal of immunology (Baltimore, Md. : 1950). 160(4) |
| ISSN: | 0022-1767 |
| Popis: | Development of CD8 alphabeta CTL epitope-based vaccines requires an effective strategy capable of co-delivering large numbers of CTL epitopes. Here we describe a DNA plasmid encoding a polyepitope or "polytope" protein, which contained multiple contiguous minimal murine CTL epitopes. Mice vaccinated with this plasmid made MHC-restricted CTL responses to each of the epitopes, and protective CTL were demonstrated in recombinant vaccinia virus, influenza virus, and tumor challenge models. CTL responses generated by polytope DNA plasmid vaccination lasted for 1 yr, could be enhanced by co-delivering a gene for granulocyte-macrophage CSF, and appeared to be induced in the absence of CD4 T cell-mediated help. The ability to deliver large numbers of CTL epitopes using relatively small polytope constructs and DNA vaccination technology should find application in the design of human epitope-based CTL vaccines, in particular in vaccines against EBV, HIV, and certain cancers. |
| Databáze: | OpenAIRE |
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