| Autor: |
M, Taira, M S, Toguchi, Y, Hamada, J, Takahashi, R, Itou, S, Toyosawa, N, Ijyuin, M, Okazaki |
| Rok vydání: |
2004 |
| Zdroj: |
Journal of materials science. Materials in medicine. 12(5) |
| ISSN: |
0957-4530 |
| Popis: |
Cytotoxicity of Ni ions on three fibroblasts such as L929, Balb/3T3 clone A31 and MC3T3-E1 were examined by cell count (CC) and Neutral Red assay (NR). Three cells were incubated for 6 days in 1 ml DME medium containing Ni ions which ranged from 0 to 2 mM/l. The results clarified that Ni ions had dose-dependent cytotoxicity. L929 possessed the largest TC50 values (the amount of Ni ion that caused 50% cell death or 50% cell viability) of 0.12 mM/l (CC) and 0.32 mM/l (NR), and Balb/3T3 clone A31 had the least values of 0.05 mM/l (CC) and 0.09 mM/l (NR), whilst MC3T3-E1 had the intermediate values of 0.08 mM/l (CC) and 0.15 mM/l (NR). The dissolution of Ni ions from Ni-containing metallic restorations must be lower than these concentration levels so that body tissues might not be severely damaged. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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