| Autor: |
N G, Seidah, D, Pélaprat, J, Rochemont, P, Lambelin, M, Dennis, J S, Chan, J, Hamelin, C, Lazure, M, Chrétien |
| Rok vydání: |
1983 |
| Předmět: |
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| Zdroj: |
Journal of chromatography. 266 |
| Popis: |
The coupling of high-performance liquid chromatography, gel-permeation, and reversed-phase chromatography with microsequencing proved to be advantageous for the unambiguous determination of specific sites in pro-opiomelanocortin, cleaved by secretory granule lysates of pig anterior pituitary. This system allows the unambiguous identification of a major chymotrypsin-like enzyme activity, optimal at pH 8.0, in the granule preparation, with a specificity directed towards some Phe decreases X and Tyr decreases X cleavage sites. The approach used emphasizes the necessity to use methodologies leading to the unambiguous determination of conversion activities. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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