Short-Term Storage of Mobilized Peripheral Blood Stem Cells in a Closed System Changes the Microenvironment and May Affect the Quantity of CD34

Autor: Amro, Elshoury, Orla, Maguire, Alexis, Conway, Joseph, Tario, Kah Teong, Soh, Maureen, Ross, Theresa, Hahn, Joanne, Becker, Paul, Wallace, Philip L, McCarthy, Hans, Minderman, George L, Chen
Rok vydání: 2022
Zdroj: Transplantation and cellular therapy.
ISSN: 2666-6367
Popis: Hypoxic conditions preserve the multipotency and self-renewing capacity of murine bone marrow and human cord blood stem cells. Blood samples stored in sealed blood gas tubes become hypoxic as leukocytes metabolize and consume oxygen. Taken together, these observations suggest that peripheral blood stem cell (PBSC) samples stored under airtight conditions become hypoxic, and that the stem cells contained may undergo qualitative or quantitative changes. This study aimed to determine the effect of storage for 8 hours in a sealed system on PBSC samples. Granulocyte colony-stimulating factor-mobilized PBSC samples were collected prospectively from 9 patients with myeloma or amyloidosis prior to apheresis, followed by measurement of CO
Databáze: OpenAIRE
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