| Autor: |
Tim H, Szeto, Pascal M W, Drake, Audrey Y-H, Teh, Nicole, Falci Finardi, Ashleigh G, Clegg, Mathew J, Paul, Rajko, Reljic, Julian K-C, Ma |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2480 |
| ISSN: |
1940-6029 |
| Popis: |
Nicotiana tabacum (the tobacco plant ) has numerous advantages for molecular farming, including rapid growth, large biomass and the possibility of both cross- and self-fertilization. In addition, genetic transformation and tissue culture protocols for regeneration of transgenic plants are well-established. Here, we describe the production of transgenic tobacco using Agrobacterium tumefaciens and the analysis of recombinant proteins, either in crude plant extracts or after purification, by enzyme-linked immunosorbent assays, sodium dodecyl sulfate polyacrylamide gel electrophoresis with western blotting and surface plasmon resonance. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
|
