[Comparing the primer specificity for betaproteobacterial ammonia-oxidizing bacteria in recirculation aquaculture systems]

Autor:	Fangfang, Pei, Peng, Zhu, Hang, Min, Kaihong, Lu, Yangfang, Ye
Rok vydání:	2012
Předmět:	DNA Bacterial Molecular Sequence Data Betaproteobacteria Aquaculture DNA Ribosomal Polymerase Chain Reaction Sensitivity and Specificity Species Specificity Ammonia RNA Ribosomal 16S Water Microbiology Oxidation-Reduction Phylogeny DNA Primers
Zdroj:	Wei sheng wu xue bao = Acta microbiologica Sinica. 51(10)
ISSN:	0001-6209
Popis:	To analyze the difference of specificity of four primers for betaproteobacterial ammonia-oxidizing bacteria (beta-AOB) 16S rDNA gene from the biofilm of closed recirculation aquaculture systems.We used 16S rDNA clone libraries to describe the beta-AOB diversity.CTO189f/CTO654r produced the highest frequency of beta-AOB-like sequences (67.3%). The amplification performance of primer was noticeably influenced by the biofilm samples. Hereinto, the biofilm of closed recirculation aquaculture systems of Tilapia nilotica resulted in the higher amplification performance of primers.CTO189f/CTO654r exhibited the highest specific for beta-AOB 16S rDNA gene from the biofilm of closed recirculation aquaculture systems.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::3bb9ef816e1d5e02c1295aaa69a44c57 https://pubmed.ncbi.nlm.nih.gov/22233055 Zobrazit plný text záznamu