| Autor: |
O E, Trubetskaia, T A, Muranova, O I, Rezikova, G V, Afanas'eva, K Iu, Reznikov |
| Rok vydání: |
1994 |
| Předmět: |
|
| Zdroj: |
Bioorganicheskaia khimiia. 20(12) |
| ISSN: |
0132-3423 |
| Popis: |
Human alpha 1-acid glycoprotein (orosomucoid, AGP) was purified to homogeneity by a two-step procedure using phenol and chloroform deproteinization of serum with subsequent preparative electrophoresis on agarose gel. It was obtained 0.15-0.3 mg of protein from 1 ml of serum. Human alpha 1-AGP binds ethidium bromide but not DNA. Neither other human serum proteins nor mice and rat alpha 1-AGP bind ethidium bromide. This property of human orosomucoid depends on its amino acid sequence rather than on the carbohydrate part of the molecule. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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