| Autor: |
Ya-Hui, Shao, Ding-Li, Xu, Ping, Zeng, Wen-Yan, Lai, Hao, Ren, Rong-Yan, Jiang |
| Rok vydání: |
2005 |
| Předmět: |
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| Zdroj: |
Di 1 jun yi da xue xue bao = Academic journal of the first medical college of PLA. 25(3) |
| ISSN: |
1000-2588 |
| Popis: |
To investigate the effects of advanced glycation end-products (AGEs) on transient cytosolic free calcium in neonatal rat cardiac myocytes (CMs) cultured in vitro.CMs cultured for 3 to 5 days in vitro were incubated with Ca(2+)-sensitive fluorescent indicator Fluo-3AM with light screening at 37 degrees celsius; with 5% CO(2) for 60 min. Changes of the fluorescence signal of free calcium caused by AGEs were measured under laser scanning confocal microscope (LSCM).Compared with the control cells, AGEs caused an increase in the concentration of cytosolic free calcium in a dose-dependent manner.AGEs may impair neonatal rat CMs by altering cytosolic free calcium concentration. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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