| Autor: |
A P, Topolyan, M A, Belyaeva, E E, Bykov, P V, Coodan, E A, Rogozhin, D A, Strizhevskaya, O M, Ivanova, A V, Ustinov, I V, Mikhura, I A, Prokhorenko, V A, Korshun, A A, Formanovsky |
| Rok vydání: |
2016 |
| Předmět: |
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| Zdroj: |
Acta Naturae |
| ISSN: |
2075-8251 |
| Popis: |
Detection of aminoglycoside antibiotics by MS or HPLC is complicated, because a) carbohydrate molecules have low ionization ability in comparison with other organic molecules (particularly in MALDI-MS), and b) the lack of aromatics and/or amide bonds in the molecules makes common HPLC UV-detectors useless. Here, we report on the application of a previously developed method for amine derivatization with tris(2,6- dimethoxyphenyl)carbenium ion to selective modification of aminoglycoside antibiotics. Only amino groups bound to primary carbons get modified. The attached aromatic residue carries a permanent positive charge. This makes it easy to detect aminoglycoside antibiotics by MS-methods and HPLC, both as individual compounds and in mixtures. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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