[Epitheliotrophic capacity of serum and plasma eyedrops. Influence of centrifugation]
| Autor: | P, Herminghaus, G, Geerling, D, Hartwig, T, Wedel, L, Dibbelt |
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| Jazyk: | němčina |
| Rok vydání: | 2004 |
| Předmět: |
Platelet-Derived Growth Factor
Serum Dose-Response Relationship Drug Epidermal Growth Factor Cell Survival Platelet Count Epithelium Corneal Centrifugation Enzyme-Linked Immunosorbent Assay Blood Proteins Fibronectins Adenosine Triphosphate Transforming Growth Factor beta Microscopy Electron Scanning Humans Ophthalmic Solutions Vitamin A Chromatography High Pressure Liquid Cell Line Transformed |
| Zdroj: | Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft. 101(10) |
| ISSN: | 0941-293X |
| Popis: | Serum eyedrops are a new modality for the treatment of ocular surface disorders. We examined the influence of the preparation of blood products in a cell culture model and compared it with plasma.Serum and plasma were obtained from full blood of ten healthy volunteers and centrifuged at 500 and 3000 G. EGF, PDGF, TGF-beta1, fibronectin, and vitamin A were quantified by means of ELISA and HPLC. Cultures of human corneal epithelial cells were incubated with the four blood products in dose-response experiments and the intracellular ATP quantified.EGF, PDGF, and vitamin A were present in serum in significantly higher concentrations than in plasma. The concentration of fibronectin was not influenced by the preparation. Support of proliferation was best by 25% platelet-poor serum. Serum supported the differentiation and migration of epithelial cells better than plasma.The biochemical character of serum eyedrops is determined by the parameters chosen to produce the blood product. Plasma does not seem to offer an epitheliotrophic capacity equivalent to serum eyedrops. Their production should be optimized before any meaningful randomized controlled clinical trial can be attempted. |
| Databáze: | OpenAIRE |
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