| Popis: |
ELISA in the sandwich modification was developed to detect anti-idiotypic antibodies to the antituberculosis idiotype of monoclonal antibodies (MAb) S4C1G4 (anti 15-18 kDa H37Rv). Sera immunoglobulins were determined by Staphylococcus protein A capture. F[ab+]2, the MAb fragments S4C1G4 (IgG2a, kappa) and 1.3.3.B5 (IgG2a, kappa), were used as a negative control, which were not recognized mycobacterial antigens and human immunoglobulins. The anti-idiotypic antibody titers were determined in the murine hyperimmune sera during the period of immunization with the MSAb SaC1G4. The method detected anti-idiotypic antibodies in the sera of infected mice and in patients with pulmonary tuberculosis. It is suggested that this method is applicable while using diagnostic tools. |
