| Autor: |
M Sh, Dvali, V P, Varlamov, G I, Kvesitadze, S V, Rogozhin |
| Rok vydání: |
1978 |
| Předmět: |
|
| Zdroj: |
Prikladnaia biokhimiia i mikrobiologiia. 14(1) |
| ISSN: |
0555-1099 |
| Popis: |
Immobilized forms of alpha-amylase from Aspergillus oryzae were prepared on the porous glass and silochrome by the glutaraldehyde method. An addition of calcium ions at a concentration of 0.05 M to the reaction mixture during immobilization stabilized the enzyme activity. pH optimum of the insoluble form of alpha-amylase was 5.8 and that of the soluble form was 4.7. Storage of the insoluble enzyme as water suspension in 0.015 M CaCl2 at 4 degrees C for six months and twenty times repeated specific reaction did not affect significantly the activity of insoluble alpha-amylase. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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