| Autor: |
Tahsin, Kuzdere, Valentin, Flury, Thomas, Schalch, Vytautas, Iesmantavicius, Daniel, Hess, Marc, Bühler |
| Rok vydání: |
2022 |
| Zdroj: |
EMBO reportsReferences. 24(1) |
| ISSN: |
1469-3178 |
| Popis: |
Methylation of histone H3 at lysine 9 (H3K9) is a hallmark of heterochromatin that plays crucial roles in gene silencing, genome stability, and chromosome segregation. In Schizosaccharomyces pombe, Clr4 mediates both di- and tri-methylation of H3K9. Although H3K9 methylation has been intensely studied in mitotic cells, its role during sexual differentiation remains unclear. Here, we map H3K9 methylation genome-wide during meiosis and show that constitutive heterochromatin temporarily loses H3K9me2 and becomes H3K9me3 when cells commit to meiosis. Cells lacking the ability to tri-methylate H3K9 exhibit meiotic chromosome segregation defects. Finally, the H3K9 methylation switch is accompanied by differential phosphorylation of Clr4 by the cyclin-dependent kinase Cdk1. Our results suggest that a conserved master regulator of the cell cycle controls the specificity of an H3K9 methyltransferase to prevent ectopic H3K9 methylation and to ensure faithful gametogenesis. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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