| Autor: |
U K, Misra, C L, Adlakha, G, Gawdi, M K, McMillian, S V, Pizzo, D T, Laskowitz |
| Rok vydání: |
2001 |
| Předmět: |
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| Zdroj: |
Journal of leukocyte biology. 70(4) |
| ISSN: |
0741-5400 |
| Popis: |
Apolipoprotein E (ApoE) is a 34-kDa cholesterol transport protein that also possesses immunomodulatory properties. In this study, we demonstrate that ApoE initiates a signaling cascade in murine peritoneal macrophages that leads to increased production of inositol triphosphate with mobilization of intracellular Ca(2+) stores. This cascade is inhibited by pretreatment with receptor-associated protein and Ni(2+), and it is mediated by a pertussis toxin-sensitive G protein. These properties are characteristic of signal transduction induced via ligand binding to the cellular receptor, lipoprotein receptor-related protein. A peptide derived from the receptor-binding region of ApoE also initiates signal transduction in a manner similar to that of the intact protein, suggesting that this isolated region is sufficient for signal transduction. The ApoE-mimetic peptide competed for binding with the intact protein, confirming that they both interact with the same site. ApoE-dependent signal transduction might play a role in mediating the functional properties of this lipoprotein. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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