Effect of heme oxygenase-1 transduced bone marrow mesenchymal stem cells on damaged intestinal epithelial cells in vitro
| Autor: | Yi, Cao, Ben-Juan, Wu, Wei-Ping, Zheng, Ming-Li, Yin, Tao, Liu, Hong-Li, Song |
|---|---|
| Rok vydání: | 2016 |
| Předmět: |
Male
Cell Survival Tumor Necrosis Factor-alpha Interleukins Bone Marrow Cells Epithelial Cells Mesenchymal Stem Cells Hematopoietic Stem Cells Mesenchymal Stem Cell Transplantation Rats Transduction Genetic Zonula Occludens-1 Protein Animals Cytokines Humans Caco-2 Cells Intestinal Mucosa Rats Wistar Heme Oxygenase-1 |
| Zdroj: | Cell biology international. 41(7) |
| ISSN: | 1095-8355 |
| Popis: | In this study, we explored the effects of mesenchymal stem cells (MSCs) from bone marrow overexpressing heme oxygenase-1 (HO-1) on the damaged human intestinal epithelial barrier in vitro. Rat MSCs were isolated from bone marrow and transduced with rat HO-1 recombinant adenovirus (HO-MSCs) for stable expression of HO-1. Colorectal adenocarinoma 2 (Caco2) cells were treated with tumor necrosis factor-α (TNF-α) to establish a damaged colon epithelial model. Damaged Caco2 were cocultured with MSCs, Ad-MSCs, Ad-HO + MSCs or HO-MSCs. mRNA and protein expression of Zona occludens-1 (ZO-1) and human HO-1 and the release of cytokines were measured. ZO-1 and human HO-1 in Caco2 were significantly decreased after treatment with TNF-α; and this effect was reduced when coculture with MSCs from bone marrow. Expression of ZO-1 was not significantly affected by Caco2 treatment with TNF-α, Ad-HO, and MSCs. In contrast, ZO-1 and human HO-1 increased significantly when the damaged Caco2 was treated with HO-MSCs. HO-MSCs showed the strongest effect on the expression of ZO-1 in colon epithelial cells. Coculture with HO-MSCs showed the most significant effects on reducing the expression of IL-2, IL-6, IFN-γ and increasing the expression of IL-10. HO-MSCs protected the intestinal epithelial barrier, in which endogenous HO-1 was involved. HO-MSCs play an important role in the repair process by reducing the release of inflammatory cytokines and increasing the release of anti-inflammatory factors. These results suggested that HO-MSCs from bone marrow were more effective in repairing the damaged intestinal epithelial barrier, and the effectiveness of MSCs was improved by HO-1 gene transduction, which provides favorable support for the application of stem cell therapy in the intestinal diseases. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text