| Autor: |
D, Colangelo, H Y, Guo, K M, Connors, T, Kubota, L, Silvestro, R M, Hoffman |
| Rok vydání: |
1992 |
| Předmět: |
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| Zdroj: |
Anticancer research. 12(5) |
| ISSN: |
0250-7005 |
| Popis: |
We have in this study used the 3-(4,5-dimethyl-2- thiazoyl) -2,5-diphenyl 2-H-tetrazolium bromide (MTT) end point in our histoculture drug-response assay. We have previously demonstrated that the formazan crystals formed by MTT reduction by mitochondrial succinate dehydrogenase reflect polarized light and can be measured by pixel analysis in intact tissue. The results described here indicate a total specificity of 93.8% and a total accuracy of 74.6% of the MTT end point for drug response in histoculture correlating with nine different human xenograft tumors grown in nude mice with respect to the in vivo drug response data. This in vitro system allows prediction of positive and negative responses to drugs, with a rate of 70% and 71.8%, respectively. The system described here has potential for clinical use because of the possibility of simultaneous description of the MTT values and heterogeneous response to drugs within individual tumors. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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