Proteomic landscape of SARS-CoV-2- and MERS-CoV-infected primary human renal epithelial cells
| Autor: | Aneesha, Kohli, Lucie, Sauerhering, Sarah K, Fehling, Kevin, Klann, Helmut, Geiger, Stephan, Becker, Benjamin, Koch, Patrick C, Baer, Thomas, Strecker, Christian, Münch |
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| Rok vydání: | 2022 |
| Předmět: |
Cell Nucleus
Proteomics Proteome SARS-CoV-2 Primary Cell Culture COVID-19 Computational Biology Epithelial Cells Kidney Virus Replication Mitochondria Kidney Tubules Proximal Gene Expression Regulation Host-Pathogen Interactions Middle East Respiratory Syndrome Coronavirus Humans Coronavirus Infections Kidney Tubules Distal Biomarkers Cells Cultured |
| Zdroj: | Life science alliance. 5(5) |
| ISSN: | 2575-1077 |
| Popis: | Acute kidney injury is associated with mortality in COVID-19 patients. However, host cell changes underlying infection of renal cells with SARS-CoV-2 remain unknown and prevent understanding of the molecular mechanisms that may contribute to renal pathology. Here, we carried out quantitative translatome and whole-cell proteomics analyses of primary renal proximal and distal tubular epithelial cells derived from human donors infected with SARS-CoV-2 or MERS-CoV to disseminate virus and cell type-specific changes over time. Our findings revealed shared pathways modified upon infection with both viruses, as well as SARS-CoV-2-specific host cell modulation driving key changes in innate immune activation and cellular protein quality control. Notably, MERS-CoV infection-induced specific changes in mitochondrial biology that were not observed in response to SARS-CoV-2 infection. Furthermore, we identified extensive modulation in pathways associated with kidney failure that changed in a virus- and cell type-specific manner. In summary, we provide an overview of the effects of SARS-CoV-2 or MERS-CoV infection on primary renal epithelial cells revealing key pathways that may be essential for viral replication. |
| Databáze: | OpenAIRE |
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