| Autor: |
E N, Liukmanova, A A, Shul'ga, D A, Arsen'eva, K A, Pluzhnikov, D A, Dolgikh, A S, Arsen'ev, M P, Kirpichnikov |
| Rok vydání: |
2004 |
| Předmět: |
|
| Zdroj: |
Bioorganicheskaia khimiia. 30(1) |
| ISSN: |
0132-3423 |
| Popis: |
Neurotoxin II from the venom of cobra Naja oxiana is a short type alpha-neurotoxin, which competitively inhibits nicotinic acetylcholine receptor. The toxin gene was expressed as a construct fused with the thioredoxin gene and the linker encoding the enteropeptidase recognition site and a Met residue between the genes. The fusion protein was mainly cleaved by cyanogen bromide, since enteropeptidase was less effective. The yield of neurotoxin II was 6 mg/l of the bacterial culture. The resulting recombinant protein was identified with native neurotoxin II by its N-terminal analysis, mass spectrometry, and NMR spectroscopy. The English version of the paper: Russian Journal of Bioorganic Chemistry, 2004, vol. 30, no. 1; see also http://www.maik.ru. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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