A new method for assaying adhesion of cancer cells to the greater omentum and its application for evaluating anti-adhesion activities of chemically synthesized oligosaccharides

Autor:	A, Okamura, S, Yazawa, T, Nishimura, S, Tanaka, I, Takai, S, Kudo, T, Asao, H, Kuwano, K L, Matta, S, Akamatsu, N, Kochibe
Rok vydání:	2001
Předmět:	Male Mice Inbred BALB C Molecular Sequence Data Oligosaccharides Cell Separation Flow Cytometry Mice Carbohydrate Sequence Colonic Neoplasms Cell Adhesion Microscopy Electron Scanning Tumor Cells Cultured Animals Neoplasm Metastasis Omentum Peritoneal Cavity
Zdroj:	Clinicalexperimental metastasis. 18(1)
ISSN:	0262-0898
Popis:	A new ex vivo method for assaying adhesion of cancer cells to the greater omentum has been developed using mouse greater omentum and [3H]labelled human gastric and mouse colorectal cancer cells. Since the adhesion rates were found to increase up to 18 h and labelled cells seemed to be stable during the period, the present method could be useful for investigating adhesion of cancer cells to the greater omentum, which must occur at the first step of the peritoneal dissemination. The adhesion of cancer cells to the greater omentum was inhibited by a series of chemically synthesized oligosaccharides and Gal beta1,3[3OMeGal beta1,4GlcNAc beta1,6]alphaBn was found to be the best inhibitor. The anti-tumor effect of this novel tetrasaccharide in vivo was shown in preliminary experiments using Balb/c mice and colon26 cells.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::39ba40db2ffc970bbc96095c28ed4c3c https://pubmed.ncbi.nlm.nih.gov/11206836 Zobrazit plný text záznamu Full text from SpringerLink