The regulatory role of vasoactive intestinal peptide in lacrimal gland ductal fluid secretion: A new piece of the puzzle in tear production
| Autor: | Berczeli, Orsolya, Szarka, Dóra, Elekes, Gréta, Vizvári, Eszter, Szalay, László, Almássy, János, Tálosi, László, Ding, Chuanqing, Tóth-Molnár, Edit |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Mice
Knockout Receptors Vasoactive Intestinal Polypeptide Type I Intracellular Space Lacrimal Apparatus Cystic Fibrosis Transmembrane Conductance Regulator Tears Animals Receptors Vasoactive Intestinal Peptide Type II Calcium Carbachol Calcium Signaling Egtazic Acid hormones hormone substitutes and hormone antagonists Research Article Chelating Agents Vasoactive Intestinal Peptide |
| Zdroj: | Molecular Vision |
| ISSN: | 1090-0535 |
| Popis: | Purpose Vasoactive intestinal peptide (VIP) is an important regulator of lacrimal gland (LG) function although the effect of VIP on ductal fluid secretion is unknown. Therefore, the aim of the present study was to investigate the role of VIP in the regulation of fluid secretion of isolated LG ducts and to analyze the underlying intracellular mechanisms. Methods LGs from wild-type (WT) and cystic fibrosis transmembrane conductance regulator (CFTR) knockout (KO) mice were used. Immunofluorescence was applied to confirm the presence of VIP receptors termed VPAC1 and VPAC2 in LG duct cells. Ductal fluid secretion evoked by VIP (100 nM) was measured in isolated ducts using videomicroscopy. Intracellular Ca2+ signaling underlying VIP stimulation was investigated with microfluorometry. Results VIP stimulation resulted in a robust and continuous fluid secretory response in isolated duct segments originated from WT mice. In contrast, CFTR KO ducts exhibited only a weak pulse-like secretion. A small but statistically significant increase was detected in the intracellular Ca2+ level [Ca2+]i during VIP stimulation in the WT and in CFTR KO ducts. VIP-evoked changes in [Ca2+]i did not differ considerably between the WT and CFTR KO ducts. Conclusions These results suggest the importance of VIP in the regulation of ductal fluid secretion and the determining role of the adenylyl cyclase-cAMP-CFTR route in this process. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|