The ability of the aryl hydrocarbon receptor to regulate ovarian follicle growth and estradiol biosynthesis in mice depends on stage of sexual maturity
| Autor: | Isabel, Hernandez-Ochoa, Kimberly R, Barnett-Ringgold, Stacey L, Dehlinger, Rupesh K, Gupta, Traci C, Leslie, Katherine F, Roby, Jodi A, Flaws |
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| Rok vydání: | 2010 |
| Předmět: |
Mice
Knockout Aging Estradiol Reverse Transcriptase Polymerase Chain Reaction Cell Count Receptors LH Mice Inbred C57BL Mice Organ Culture Techniques Ovarian Follicle Receptors Aryl Hydrocarbon Receptors Androgen Basic Helix-Loop-Helix Transcription Factors Oocytes Animals Cyclin D2 Receptors FSH Female RNA Messenger Sexual Maturation Testosterone Congeners Research Article |
| Zdroj: | Biology of reproduction. 83(5) |
| ISSN: | 1529-7268 |
| Popis: | The aryl hydrocarbon receptor (AHR) is a ligand-activated transcription factor that mediates the toxicity of environmental chemicals and regulates many physiological functions, including processes in female reproduction. Previous studies demonstrated that Ahr deletion leads to slow ovarian follicle growth because of impaired estradiol production and reduced gonadotropin responsiveness in prepubertal mice. These studies, however, did not determine how Ahr deletion impairs estradiol production or whether the effects of Ahr deletion on follicle growth and estradiol production persist in adulthood. Thus, the present study evaluated the effect of Ahr deletion on steroid precursors in the estradiol biosynthesis pathway. Furthermore, this study evaluated follicle growth and estradiol biosynthesis in wild-type (WT) and Ahr knockout (AhrKO) antral follicles at different stages of sexual maturity. AhrKO antral follicles from prepubertal mice had slower growth, produced lower estradiol levels, and had reduced cyclin D2 (Ccnd2) expression compared to WT follicles. AhrKO follicles from adult mice, however, produced higher androgen levels and expressed higher levels of Ccnd2 compared to WT follicles. Furthermore, AhrKO follicles from adult mice had growth to that of WT follicles. These findings suggest that the AHR regulates follicle growth by altering factors involved in the estradiol biosynthesis pathway as well as key regulators of follicle growth and that this role of AHR depends on stage of sexual maturity. |
| Databáze: | OpenAIRE |
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