Polyclonal antibodies to a recombinant coat protein of Potato virus A

Autor:	N, Cerovská, T, Moravec, J, Velemínský
Rok vydání:	2003
Předmět:	Microscopy Electron Blotting Western Potyvirus Escherichia coli Antibodies Monoclonal Capsid Proteins Enzyme-Linked Immunosorbent Assay Cloning Molecular Antibodies Viral Recombinant Proteins Solanum tuberosum
Zdroj:	Acta virologica. 46(3)
ISSN:	0001-723X
Popis:	Specific mouse antibodies against a recombinant coat protein (CP) of Potato virus A (PVA) were produced. The PVA CP gene was cloned in an expression vector pMPM4omega. After expression in Escherichia coli the presence of the expressed CP was proved by Western blot analysis using polyclonal and monoclonal antibodies (MAbs). The expressed CP was purified by centrifugation in CsCl density gradient or on a sucrose cushion. The production of virus-like particles (VLPs) was proved by electron microscopy. The purified CP was used for preparation of a mouse antiserum which had a titer of 1:1024 in ELISA and reacted specifically in Western blot analysis and indirect plate-trapped antigen ELISA (PTA-ELISA).
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::398a1caa80a6e14da7262d185da26100 https://pubmed.ncbi.nlm.nih.gov/12580376 Zobrazit plný text záznamu