Autor: |
Martin, Hartmann, Charles G, Howes, Vilmar, Veldre, Salome, Schneider, Parag A, Vaishampayan, Anthony C, Yannarell, Christopher, Quince, Per, Johansson, K Johanna, Björkroth, Kessy, Abarenkov, Steven J, Hallam, William W, Mohn, R Henrik, Nilsson |
Rok vydání: |
2011 |
Předmět: |
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Zdroj: |
FEMS microbiology letters. 319(2) |
ISSN: |
1574-6968 |
Popis: |
Reverse complementary DNA sequences - sequences that are inadvertently given backwards with all purines and pyrimidines transposed - can affect sequence analysis detrimentally unless taken into account. We present an open-source, high-throughput software tool -v-revcomp (http://www.cmde.science.ubc.ca/mohn/software.html) - to detect and reorient reverse complementary entries of the small-subunit rRNA (16S) gene from sequencing datasets, particularly from environmental sources. The software supports sequence lengths ranging from full length down to the short reads that are characteristic of next-generation sequencing technologies. We evaluated the reliability of v-revcomp by screening all 406 781 16S sequences deposited in release 102 of the curated SILVA database and demonstrated that the tool has a detection accuracy of virtually 100%. We subsequently used v-revcomp to analyse 1 171 646 16S sequences deposited in the International Nucleotide Sequence Databases and found that about 1% of these user-submitted sequences were reverse complementary. In addition, a nontrivial proportion of the entries were otherwise anomalous, including reverse complementary chimeras, sequences associated with wrong taxa, nonribosomal genes, sequences of poor quality or otherwise erroneous sequences without a reasonable match to any other entry in the database. Thus, v-revcomp is highly efficient in detecting and reorienting reverse complementary 16S sequences of almost any length and can be used to detect various sequence anomalies. |
Databáze: |
OpenAIRE |
Externí odkaz: |
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