| Autor: |
P J, Dailey, M L, Collins, M S, Urdea, J C, Wilber |
| Rok vydání: |
2011 |
| Zdroj: |
Methods in molecular medicine. 19 |
| ISSN: |
1543-1894 |
| Popis: |
With this statement, Sherlock and Dooley have described two of the three major challenges involved in quantitatively measuring any analyte in tissue samples: the distribution of the analyte in the tissue; and the standard of reference, or denominator, with which to make comparisons between tissue samples. The third challenge for quantitative measurement of an analyte in tissue is to ensure reproducible and quantitative recovery of the analyte on extraction from tissue samples. This chapter describes a method that can be used to measure HCV RNA quantitatively in liver biopsy and tissue samples using the bDNA assay. All three of these challenges-distribution, denominator, and recovery-apply to the measurement of HCV RNA in liver biopsies. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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