| Autor: |
Shweta, Sharma, Tej Krishan, Bhat, Munishwar Nath, Gupta |
| Rok vydání: |
2002 |
| Předmět: |
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| Zdroj: |
Biotechnology and applied biochemistry. 35(3) |
| ISSN: |
1470-8744 |
| Popis: |
Tannase from Aspergillus niger van Teighem was immobilized on concanavalin A-Sepharose via bioaffinity interaction. The immobilized enzyme showed a pH optimum similar to that of the free enzyme. K(m) values for free and immobilized enzyme were 0.3 and 0.6 mM respectively. V(max) changed from 0.013 to 0.02 micromol x min(-1) upon immobilization. The immobilized preparation was quite stable to reuse, there was no loss of enzyme activity after three cycles and it retained 81% activity even after the sixth cycle. Ester hydrolysis using the immobilized enzyme led to a 40% conversion into gallic acid as compared with 30% obtained with the free enzyme. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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