| Autor: |
Domenico, Rizzo, Daniele, Da Lio, Linda, Bartolini, Cristina, Francia, Antonio, Aronadio, Nicola, Luchi, Sara, Campigli, Guido, Marchi, Elisabetta, Rossi |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
Methods in molecular biology (Clifton, N.J.). 2536 |
| ISSN: |
1940-6029 |
| Popis: |
DNA extraction from plant samples is very important for a good performance of diagnostic molecular assays in phytopathology. The variety of matrices (such as leaves, roots, and twigs) requires a differentiated approach to DNA extraction. Here we describe three categories of matrices: (a) symptomatic bark/wood tissue; (b) residues of frass resulting from insect woody trophic activities, portions of the galleries produced in the wood, and tissues surrounding exit holes; and (c) leaves of different plant species. To improve the performances of diagnostic assays, we here describe DNA extraction procedures that have been optimized for each matrix type. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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