Expression of human interferon alpha H, an interferon with two potential asparagine-linked glycosylation sites

Autor:	X X, Zhao, B L, Daugherty, B, Schwartz, S, Maeda, S, Pestka
Rok vydání:	1987
Předmět:	DNA Bacterial Glycosylation Base Sequence Genetic Vectors Oligonucleotides Recombinant Proteins Gene Expression Regulation Interferon Type I Escherichia coli Humans Asparagine Chromosome Deletion Phosphorylation Plasmids
Zdroj:	Journal of biological regulators and homeostatic agents. 1(3)
ISSN:	0393-974X
Popis:	Of the large number of human alpha interferon genes identified, only one, Hu-IFN-alpha H, contains potential asparagine-linked glycosylation sites. With the use of a new vector that permits convenient expression, site-specific mutation, and DNA sequencing, Hu-IFN-alpha H was expressed in Escherichia coli. The bacterial product which is not glycosylated is fully active demonstrating that the carbohydrate on this species is not required for antiviral activity.
Databáze:	OpenAIRE
Externí odkaz:	https://explore.openaire.eu/search/publication?articleId=pmid________::386b962dbc38ac7059e87ad40ec314ca https://pubmed.ncbi.nlm.nih.gov/3332544 Zobrazit plný text záznamu