| Autor: |
Karen, Gomez-Mariscal, Belinda C, Gómez-Meda, Ana L, Zamora-Perez, María G, Sánchez-Parada, Martha P, Gallegos-Arreola, Guillermo M, Zúñiga-González |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Annals of clinical and laboratory science. 48(2) |
| ISSN: |
1550-8080 |
| Popis: |
The aim of the study was to determine if amniotic fluid cells of rats can be used to provide evidence of genotoxicity. In order to do that micronuclei formation was induced in rats during pregnancy after treatment with cyclophosphamide (CP), at different CP doses. On gestational day 19, we collected the amniotic fluid and determined the frequency of micronucleated cells (MNCs) from the offspring. Samples were centrifuged and placed on clean slides. The smears were observed with an epifluorescence microscope. The number of MNCs in 2000 cells per pregnant rat was counted. The fetus weight and size were recorded and provided evidence of DNA damage caused by CP administration to their mothers. A significantly greater number of MNCs was observed only for the medium CP dose ( |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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