Indirect regeneration and genetic fidelity analysis of acclimated plantlets through SCoT and ISSR markers in
| Autor: | Gulab Khan, Rohela, Phanikanth, Jogam, Mohammad, Yaseen Mir, Aftab Ahmad, Shabnam, Pawan, Shukla, Sadanandam, Abbagani, Azra Nahaid, Kamili |
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| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Biotechnology Reports |
| ISSN: | 2215-017X |
| Popis: | Highlights • A reliable protocol developed for micropropagation of Morus alba L.cv. Chinese white through indirect regeneration using different explants. • Friable callus was induced in maximum amounts from leaf and nodal explants on MS media supplemented with 2,4-D and NAA respectively. • Highest frequency of regeneration was obtained from the leaf and node derived callus on MS media with BAP + TDZ and plantlets were rooted on IBA. • Genetic homogeneity of in vitro raised plants was confirmed by SCoT and ISSR primers based molecular analysis. A reliable protocol was developed for in vitro micro propagation of Morus alba L.cv. Chinese white. Initially, friable callus was induced (242.8 and 128.5 mg) from in vivo leaf and nodal explants on Murashige and Skoog’s (MS) medium amended with 4.0 μM/L of 2,4-Dichlorophenoxyacetic acid (2,4-D) and 3.0 μM/L of Naphthalene acetic acid (NAA) respectively within 3 weeks. Shoot regeneration (12.2 and 8.6) was obtained from leaf and node derived callus on 6-benzylaminopurine (BAP) + Thidiazuron (TDZ) at 2.5 + 2.0 and 7.5 + 2.0 μM/L concentrations respectively, after 4 weeks of incubation. In vitro shoots were rooted (90 %) on half strength MS medium with 7.5 μM/L indole-3 butyric acid (IBA) and plantlets were hardened in plastic pots contained farmyard manure, sand and garden soil in 1:1:2 ratio. The genetic stability of plantlets were confirmed by start codon targeted (SCoT) and inter simple sequence repeats (ISSR) primers based molecular analysis. |
| Databáze: | OpenAIRE |
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