[Chemical constituents of Rabdosia japonica var. glaucocalyx and their anti-complementary activity]
| Autor: | Shi, Yao, Nai-Yu, Xu, Chun-Jun, Chu, Jian, Zhang, Dao-Feng, Chen |
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| Rok vydání: | 2013 |
| Předmět: |
Male
Chromatography Spectrometry Mass Electrospray Ionization Erythrocytes Magnetic Resonance Spectroscopy Sheep Esters Ethylenes Plant Components Aerial Complement Hemolytic Activity Assay Antioxidants Caffeic Acids Complement Inactivating Agents Plant Growth Regulators Cricetinae Isodon Animals Female Chromatography High Pressure Liquid Drugs Chinese Herbal |
| Zdroj: | Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. 38(2) |
| ISSN: | 1001-5302 |
| Popis: | To study the chemical constituents of Rabdosia japonica var. glaucocalyx and their anti-complementary activity on the basis of preliminary studies. Target isolation guided by anti-complementary activity test, compounds in the chloroform and n-butanol fractions were isolated and purified by silica gel and Sephadex LH-20 column chromatographies, and preparative HPLC. The structures were identified by various spectroscopic data including ESI-MS, 1H-NMR and 13C-NMR data. The compounds were evaluated for anti-complementary activity in vitro. Eleven compounds were isolated from the chloroform and n-butanol soluble fractions and identified as stigmasterol (1), stigmas-9 (11) -en-3-ol (2), glaucocalyxin D (3), kamebakaurin (4), maslinic acid (5), corosolic acid (6), minheryins I (7), diosmetin (8), caffeic acid ethylene ester (9), caffeic acid (10) and vitexin (11). Isoquercetrin, rutin, quercetin, 3-methylquercetin, luteolin, 7-methylluteolin, and apigenin which were isolated from the preliminary studies together with compounds 9 and 10 showed inhibition of the complement system by the classical pathway. Compounds 2, 4, 6-9 and 11 were obtained from this plant for the first time. Caffeic acid (10) showed the strongest activity in vitro with a CH50 value of 0.041 g x L(-1). |
| Databáze: | OpenAIRE |
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