| Autor: |
Rong-Hua, Chen, Nan, Liu, De-Shan, Liu, Hou-Wei, DU, Yi-Xian, Zhang, Hua-Pin, Huang |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology. 26(10) |
| ISSN: |
1007-8738 |
| Popis: |
to explore the effects of IL-10 on the expression of interferon regulatory factor-1 (IRF-1) after anoxia-reoxygenation in vitro.brain microvascular endothelial cells (BMEC) and neutrophil (PMN) were co-cultured exposed to eight hours of anoxia followed by two hours of reoxygenation to establish anoxia-reoxygenation model in vitro. The co-cultured BMEC and PMN were randomly divided into the following groups: normal group, anoxia-reoxygenation group, 1 microg/L IL-10 treatment group, 10 microg/L IL-10 treatment group, and 30 microg/L IL-10 treatment group. The expression of IRF-1 was detected by RT-PCR and Western blot method.the expression of IRF-1 was significantly upregulated after anoxia-reoxygenation. IL-10 inhibited the expression of IRF-1 in a dose-dependent manner compared with anoxia-reoxygenation group, and the peak of inhibitive effects occurred at the dose of 30 μg/L.IL-10 can inhibit the expression of interferon regulatory factor-1 with anoxia-reoxygenation in vitro. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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