| Autor: |
Dong-Ping, Wang, Wei, Shi, Wan-Wen, Ge, Ju-Xia, Zhang, Lian-Ping, Zhao, Xue, Chen, Li, Dong, Yan-Qing, Sun |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Zhongguo shi yan xue ye xue za zhi. 27(6) |
| ISSN: |
1009-2137 |
| Popis: |
To investigate the apoptosis of CD34CD34The proportion of CD34FA-2-b-β can regulate proliferation and apoptosis KG1a stem cells, the involved mechanism may be related with the activation of mitochondrial-mediated apoptotic pathway.秦巴硒菇提取物FA-2-b-β诱导CD34探讨秦巴硒菇提取物FA-2-b-β对CD34用免疫磁珠分选法从KG1a细胞株中分选出CD34经过免疫磁珠分选后KG1a干细胞比例为（95.35±2.63）%。FA-2-b-β对KG1a干细胞的增殖具有明显抑制作用，呈时间和剂量依赖性（24 h，r=0.943；48 h，r=0.976）。流式细胞术检测显示，不同浓度FA-2-b-β作用KG1a干细胞24 h时，随药物浓度增加凋亡率也随之增高。Western blot检测显示，凋亡相关蛋白BAX和Casepase-3表达上调，BCL-2和Cyclin D1的表达下调.秦巴硒菇提取物FA-2-b-β能够调控KG1a干细胞的增殖和凋亡，其抗肿瘤效应可能通过调控线粒体介导的凋亡途径实现. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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