| Autor: |
M, Pizzi, I, Sarnico, F, Boroni, A, Benetti, M, Benarese, P F, Spano |
| Rok vydání: |
2005 |
| Předmět: |
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| Zdroj: |
Acta neurochirurgica. Supplement. 93 |
| ISSN: |
0065-1419 |
| Popis: |
NF-kappaB is a nuclear transcription factor involved in the control of fundamental cellular functions including regulation of cell survival. We investigated NF-kappaB activation induced by two opposing modulators of cell viability: IL-1beta and glutamate. We found that IL-1beta activated p50, p65 and c-Rel subunits of NF-kappaB, while glutamate activated only p50 and p65 proteins. Cell stimulation by glutamate, correlated with expression of the pro-apoptotic genes Caspase-3, Caspase-2L and Bax. Conversely, IL-1beta induced the expression of the short anti-apoptotic isoform of Caspase-2. Finally, we analysed the effect of the inhibition of IkappaBalpha degradation on glutamate-induced toxicity by using BAY 11-7082, a selective inhibitor of IkappaBalpha phosphorylation. Our results suggest that BAY 11-7082 preserves neuron viability from the glutamate-mediated injury. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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