| Autor: |
Peter M, Mbelele, Christian, Utpatel, Elingarami, Sauli, Emmanuel A, Mpolya, Beatrice K, Mutayoba, Ivan, Barilar, Viola, Dreyer, Matthias, Merker, Margaretha L, Sariko, Buliga M, Swema, Blandina T, Mmbaga, Jean, Gratz, Kennedy K, Addo, Michel, Pletschette, Stefan, Niemann, Eric R, Houpt, Stellah G, Mpagama, Scott K, Heysell |
| Rok vydání: |
2021 |
| Zdroj: |
JAC-antimicrobial resistance. 4(2) |
| ISSN: |
2632-1823 |
| Popis: |
Rifampicin- or multidrug-resistant (RR/MDR)We compared WGS-based drug resistance-predictive mutations for 42 MTBC isolates from MDR-TB patients in Tanzania with the MICs of 14 antibiotics measured in the Sensititre™ MycoTB assay. An isolate was phenotypically categorized as resistant if it had an MIC above the epidemiological-cut-off (ECOFF) value, or as susceptible if it had an MIC below or equal to the ECOFF.Overall, genotypically non-wild-type MTBC isolates with high-level resistance mutations (gNWT-R) correlated with isolates with MIC values above the ECOFF. For instance, the median MIC value (mg/L) for rifampicin-gNWT-R strains was4.0 (IQR 4.0-4.0) compared with 0.5 (IQR 0.38-0.50) in genotypically wild-type (gWT-S,WGS-based drug resistance prediction worked well to rule-in phenotypic drug resistance and the absence of second-line drug resistance-mediating mutations has the potential to guide the design of RR/MDR-TB regimens in the future. |
| Databáze: |
OpenAIRE |
| Externí odkaz: |
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