Misidentification of Cutibacterium namnetense as Cutibacterium acnes among clinical isolates by MALDI-TOF VitekMS: usefulness of gyrB sequencing and new player in bone infections

Autor: L, Ruffier d'Epenoux, N, Arshad, P, Bémer, M-E, Juvin, G, Le Gargasson, A, Guillouzouic, S, Corvec
Rok vydání: 2020
Předmět:
Zdroj: European journal of clinical microbiologyinfectious diseases : official publication of the European Society of Clinical Microbiology. 39(8)
ISSN: 1435-4373
Popis: The taxonomy modification of Propionibacterium sp. with the description of new species, especially Cutibacterium namnetense, raises the question of species distribution in routine clinical samples. We performed a retrospective study during 3 years before the implementation of MALDI-TOF. Two hundred sixty-nine isolates were included in the study. MALDI-TOF identification, 16S rRNA, and new developed gyrB partial sequencings were performed. The most representative species was C. acnes in 88% of the cases, regardless of the origin of the clinical sample. Eventually, we identified three C. namnetense strains, representing a 1.1% prevalence over the period of time, including two bone infections. MALDI-TOF databases should be regularly updated to incorporate new species. gyrB sequencing constitutes a both easy and relevant method to identify Cutibacterium sp. especially C. namnetense, a new player in bone infections.
Databáze: OpenAIRE