[Synthesis of alpha-fetoprotein in primary cultures of hepatocytes from adult mice]

Autor:	A S, Gleĭberman
Rok vydání:	1982
Předmět:	Immunoenzyme Techniques Mice Inbred C57BL Mice Mice Inbred BALB C Time Factors Liver Albumins Animals alpha-Fetoproteins Cells Cultured
Zdroj:	Biulleten' eksperimental'noi biologii i meditsiny. 93(1)
ISSN:	0365-9615
Popis:	Alpha-fetoprotein (AFP) and albumin synthesis in primary hepatocyte cultures of adult mice was studied by the immunoperoxidase techniques. Albumin was detectable in all hepatocytes during the cultivation period (from day 1 to day 8). AFP could be found regularly beginning from days 3-4 up to day 8. Attempts to obtain hepatocytes surviving for a longer period of time ended in failure. The number of AFP-containing hepatocytes in the culture ranged within single cells to about half of all the hepatocytes. The proliferating hepatocytes that contained both AFP and albumin were found in some of the experiments.
Databáze:	OpenAIRE
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